Origin of hydrogen in methane produced by Methanobacterium thermoautotrophicum.

نویسندگان

  • L Daniels
  • G Fulton
  • R W Spencer
  • W H Orme-Johnson
چکیده

The production of deuterated methane by Methanobacterium thermoautotrophicum in H2O-D2O mixtures was examined by high-resolution mass spectrometry. The hydrogen in the methane arose solely from water and not from hydrogen gas. Hydrogen gas served only as an electron source in methanogenesis. A whole-cell product isotope discrimination of 1.5 favoring hydrogen over deuterium was observed in methane production in 81 atom% deuterated water. The distribution of deuterated methane species is described by a simple model of the overall reaction.

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عنوان ژورنال:
  • Journal of bacteriology

دوره 141 2  شماره 

صفحات  -

تاریخ انتشار 1980